Application of light incubator in the study of apple virus disease prevention and treatment
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At present, the combination of heat treatment and shoot tip culture is currently the most effective detoxification method on apples, and the light incubator is mainly used for the heat treatment process, but due to heat treatment will lead to reduced proliferation coefficient, small growth, low survival rate and Problems such as inconvenience in drawing materials and the like, and the characteristics of yellowing, tenderness, and rapid growth of plants in continuous dark culture conditions. Therefore, in the experiment, apple plantlets were used as test materials to explore the effect of dark culture on the growth and survival rate of the heat-treated incubator in order to further improve the efficiency of virus removal. The test process is as follows:
1 The apple tissue culture seedlings were placed in a light incubator for light culture. The starting temperature was 26/25°C, and the temperature was increased by 1°C per day. The temperature was raised to 38/32°C and cultured at a temperature of 30 days.
(2) Incubate the tissue culture seedlings in the dark in the culture room for 7 days in the light incubator dark culture. The starting temperature is 26/25°C, increase 1°C per day, rise to 38/32°C and culture at 30°C for 30 days.
3 The tissue culture seedlings were placed in a dark culture in a light incubator. The starting temperature was 26/25°C, and the temperature was increased by 1°C per day. The temperature was raised to 38/32°C and cultured at a temperature of 30 days.
4 The tissue culture seedlings were directly placed in a light incubator and incubated at 38/32°C for 30 days under warm dark conditions.
(5) Incubate the 7d cultured plantlets in the culture room directly in a light incubator and incubate at 38/32°C for 30 days.
After treatment with a light incubator, the results showed that the survival rate of the heat treatment + dark culture, the height of the surviving seedlings, and the proliferation coefficient were superior to those of the heat treatment + light culture. Respectively: treatment 1:46%, 2.1 cm, 2.1; treatment 2: 65%, 3.1 cm, 4.7; treatment 3: 83%, 3.0 cm, 5.6; treatment 4: 81%, 2.7 cm, 7.2; 70%, 3.7 cm, 6.4.
