Determination of Ammonium Salt Content in Soy Sauce by Automatic Nitrogen Determinator

Soy sauce made from wheat, soybeans, salt, water, etc., is an indispensable seasoning in people's daily life. In the process of brewing soy sauce, the sugar in the raw material will produce a small amount of acid through aspergillus, while the protein is broken down into various amino acids. The determination of ammonium in soy sauce has two very important significance. On the one hand, the content of ammonium in soy sauce needs a certain amount of control and cannot be excessive; on the other hand, it can be correctly evaluated by the determination of ammonium salt. The true result of amino nitrogen determination by the nitrogen method. The automatic nitrogen determination instrument has the functions of automatic distillation, absorption, titration, data processing, and result printing. The operation is simple, rapid, and the result is accurate and reliable. The experimental measurement is automated, and the working efficiency is effectively improved. Determination of ammonium salts in soy sauce is very worthwhile using an automatic nitrogen analyzer. To this end, some experiments were carried out to demonstrate the advantages of automatic determination of ammonium by ammonium determination.

Take 2ml sample and add 150ml water and 19magnesia to the digestive tube, place it directly on the automatic nitrogen determination instrument for distillation test, and do blank test at the same time. The entire measurement process is automatically performed from distillation, titration, data processing and result printing. Take 6 different kinds of samples, add a certain amount of sulfuric acid according to the test, and carry out the standard recovery test. The recovery rate of the added standard is between 90% and 102.9%, and the average recovery rate is 97.8%. Claim. For different kinds of samples, salt content was determined by semi-micro determination method and automatic nitrogen determination instrument. The t-test, P>0.05, proved that there was no significant difference between the two methods, and met the requirements of the method.

Boric acid absorption liquid plus human indicator must be accurate, otherwise it will affect the end of titration. According to the preparation method required by automatic azotometer, 1L boric acid absorption solution was added with 10ml 1g/L acetochlor green indicator and 10ml 1 year old L-methyl red indicator. To judge whether the preparation is accurate, it is advisable to take 25ml of boric acid solution with 100ml of distilled water and mix. The solution should be blue-gray. If it is still red, use a 1mol/L concentration of sodium hydroxide solution to adjust the boric acid solution to blue-gray. until. According to the principle of reaction, barium sulfate can be used as a standard substance for recovery.

Through rigorous scientific experiments, the use of automatic nitrogen determination of salt in soy sauce, has many advantages, the first operation is simple, fast, with a high degree of precision accuracy, a sample can be read directly in just 6min The results are applicable to the determination of large quantities of samples, and the automatic nitrogen determination instrument is worthy of popularization and application.

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